Recombinant human GDF3 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 124 amino acids and having a total molecular mass of 14.15 kDa. GDF3 is fused to a 10 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms: Growth Differentiation Factor 3, Growth/Differentiation Factor 3, MCOPCB6, MCOP7, GDF-3, KFS3.
Formulation: The protein was lyophilized from a concentrated solution (0.5 mg/ml) containing 30 mM Acetate buffer pH 4.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHAS AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDECGCG.
Uniprot ACC number: Q9NR23
Transportation method: Shipped at room temperature.
Stability: Lyophilized GDF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF3 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized GDF3 in sterile 100 mM Acetate buffer pH 4 at a concentration of 0.5 mg/ml. For the dilution into higher pH values, it is recommended to dilute the protein to a concentration of 10μg/ml. Please note that in higher concentrations the solubility of GDF3 is limited. The protein is not sterile! Please sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com