Recombinant human Granulocyte Macrophage Colony Stimulating Factor produced in Yeast is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 26-32 kDa. rhGMCSF differs from the natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. GM-CSF is purified by proprietary chromatographic techniques.
Synonyms: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-Alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution containing 10 mM phosphate buffer pH 7.0, 40 mg mannitol and 10 mg sucrose.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.
Source: Pichia Pastoris.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
Uniprot ACC number: P04141
Transportation method: Shipped at room temperature.
Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.183 ng/ml, corresponding to a Specific Activity of 5, 500,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com