Recombinant human Granulocyte Colony Stimulating Factor produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 kDa. The Pegylated G-CSF is produced by attaching a 20 kDa methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF giving a total molecular mass of 38.8 kDa. G-CSF is purified by proprietary chromatographic techniques.
Synonyms: CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
Formulation: G-CSF is supplied in solution (0.69mg/ml) containing 10 mM Acetate Buffer (pH 4.0), and 0.004% Polysorbate 80.
Physical Appearance: Colorless, clear and transparent solution.
Purity: Greater than 95% as determined by SEC-HPLC.
Source:Escherichia coli.
Uniprot ACC number: P09919
Transportation method: Shipped with Ice Packs.
Stability: G-CSF PEG should be stored refrigerated at 2° to 8°C. Vials should be kept in theirpackaging to protect from light until the time of use. Shaking and freezing should be avoided.
Biological Activity: The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml , corresponding to a Specific Activity of 10,000,000 IU/mg.
Safety data sheet: Inquire at info@3hbiomedical.com