Recombinant human gAcrp30 produced in
E. coli is a non-glycosylated, Polypeptide chain containing 145 amino acids and having a molecular mass of 16.7 kDa. The gAcrp30 is purified by proprietary chromatographic techniques.
Synonyms: Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Formulation: Sterile filtered and lyophilized from 10 mM sodium phosphate & 0.5 mM DTT, pH 7.5.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN.
Uniprot ACC number: Q15848
Transportation method: Shipped at room temperature.
Stability: For long term, store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time two weeks.
Biological Activity: gAcrp30 activity is determined by its ability to inhibit the proliferation of mouse M1 cells. The expected ED50 for this effect is 669 ng/ml, corresponding to a specific activity of 1.5x103units/mg.
Solubility: We recommended reconstituting gAcrp30 at a concentration of 0.1mg per ml with 10 mM sodium phosphate & 0.5 mM DTT, pH 7.5. which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com