Recombinant human Fibroblast Growth Factor-2 (FGF-2) produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17.3 kDa. The FGF-basic is purified by proprietary chromatographic techniques.
Synonyms: Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Formulation: The sterile protein solution (0.5 mg/ml) contains 20 mM Tris pH 7.9, 100 mM KCl, 1 mM DTT and 20% glycerol.
Physical Appearance: Sterile Filtered liquid formulation.
Purity: Greater than 98% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Baculovirus.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile.
Uniprot ACC number: P09038
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml
, corresponding to a specific activity of 2MU/mg.
Safety data sheet: Inquire at info@3hbiomedical.com