Recombinant human Fibroblast Growth Factor-2 (FGF-2) produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.2 kDa. The FGF-b is purified by proprietary chromatographic techniques.
Synonyms: Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution in 20 mM Tris-HCl, pH 7.4 and 1M NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDpH IK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.
Uniprot ACC number: P09038
Transportation method: Shipped at room temperature.
Stability: Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine balb/c 3T3 cells is <0.1 ng/ml, corresponding to a specific activity of greater than 1.0x10
7 Units/mg.
Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Activation of the Hedgehog pathway in pilocytic astrocytomasPublication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.109 3/neuonc/noq026 First published online: March 11, 2010 Link:http://neurooncology. oxfordjournals. org/content/12/8/790. full2. Title:pH enotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013. Link:http://jcs. biologists. org/content/121/7/1002. full3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain . Publication: Cell Transplantation ISSN: 0963-6897 DOI: 10.3727/096368910X498944 Volume 19, Issue 8, pages 985-998 Copyright © 2010 Cognizant Co mM. CorpLink:http://www. ingentaconnect. com/content/cog/ct/2010/00000019/00000008/art000074. Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues. Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press. Link:http://onlinelibrary. wiley. com/doi/10.1634/stemcells.2007-0979/full5. Title: Successful implantation of pH ysiologically functional bioengineered mouse internal anal spH incterPublication: American Journal of pH ysiology-Gastrointestinal and Liver pH ysiology 299.2 (2010): G430-G439. Link: http://ajpgi. pH ysiology. org/content/299/2/G430
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