Recombinant human Fibroblast Growth Factor-2 (FGF-2) produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.5 kDa. The FGF2 is purified by proprietary chromatographic techniques.
Synonyms: Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Formulation: The bFGF was lyophilized from a sterile filtered solution containing 20 mM Tris-HCl, pH 7.6 and 150 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS.
Uniprot ACC number: P09038
Transportation method: Shipped at room temperature.
Stability: Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGFb should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is < 0.05 ng/ml corresponding to a specific activity of 2.0x107 units/mg.
Solubility: It is recommended to reconstitute the lyophilized FGF-B in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com