Recombinant human Fibroblast Growth Factor-1 (FGF-1) produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15.8 kDa. The FGF-a is purified by proprietary chromatographic techniques.
Synonyms: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.
Formulation: The sterile protein solution (1.8mg/ml) contains 20 mM Tris HCl pH 7.9, 100 mM KCl, 0.2 mM DTT and 20% glycerol.
Physical Appearance: Sterile Filtered liquid formulation.
Purity: Greater than 95% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Baculovirus.
Amino acid sequence: The sequence of the first five N-terminal amino acids as determined and was found to be Met-pH e-Asn-Leu-Pro.
Uniprot ACC number: P05230
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10 ng/ml, corresponding to a specific activity of 100,000 IU/mg.
Safety data sheet: Inquire at info@3hbiomedical.com