Recombinant human Fibroblast Growth Factor-acidic produced in
E. coli is a single, non-glycosylated polypeptide chain containing 154 amino acids and having a molecular mass of 17.3 kDa. The FGF1 is purified by proprietary chromatographic techniques.
Synonyms: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.
Formulation: lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 0.5 mM DTT, 2 mM EDTA, and 5 % Trehalose.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: AEGEITTFTA LTEKFNLPPG NYKKPKLLYC SNGGHFLRIL PDGTVDGTRD RSDQHIQLQL SAESVGEVYI KSTETGQYLA MDTDGLLYGS QTPNEECLFL ERLEENHYNT YISKKHAEKN WFVGLKKNGS CKRGPRTHYG QKAILFLPLP VSSD.
Uniprot ACC number: P05230
Transportation method: Shipped at room temperature.
Stability: Lyophilized FGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-acidic should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is < than 0.5 ng/ml
, corresponding to a specific activity of > 2.0 ×106 IU/mg in the presence of 10µg/ml Heparin.
Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic Recombinant in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com