Recombinant human Fibroblast Growth Factor-9 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids and having a molecular mass of 23.4 kDa. The FGF-9 is purified by proprietary chromatographic techniques.
Synonyms: GAF (Glia-activating factor), HBGF-9, MGC119914, MGC119915, FGF-9.
Formulation: The sterile protein powder is lyophilized from 1mg/ml solution containing 1xPBS.
Physical Appearance: Sterile Filtered white Lyophilized powder.
Purity: Greater than 95% as determined by RP-HPLC and SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAVTDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIAVGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSNLYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELYKDILSQS.
Uniprot ACC number: P31371
Transportation method: Shipped at room temperature.
Stability: Lyophilized Fibroblast Growth Factor 9 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-9 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg.
Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-9 Human Recombinant sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com