Recombinant human FGF-8 produced in HEK cells is a glycosylated monomer, having a molecular weight range of 30-45 kDa due to glycosylation. The FGF8 is purified by proprietary chromatographic techniques.
Synonyms: FGF8B, FGF-8B, FGF8-B, KAL6, HBGF-8, HBGF8, AIGF, HBGF-8, MGC149376, fibroblast growth factor 8.
Formulation: The FGF-8 was Lyophilized in 10 mM Tris-HCl pH 7.4 and 800 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Uniprot ACC number: P55075
Transportation method: Shipped at room temperature.
Stability: Lyophilized FGF-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF8 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line, the ED50 is <60 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized FGF8 in sterile water containing 0.1% endotoxin-free recombinant HSA.
Safety data sheet: Inquire at info@3hbiomedical.com