Recombinant human FGF 8 produced in
E. coli is a non-glycosylated polypeptide chain containing 194 amino acids and having a total molecular mass of 22.5 kDa.
Synonyms: FGF8B, FGF-8B, FGF8-B, KAL6, HBGF-8, HBGF8, AIGF, HBGF-8, MGC149376, fibroblast growth factor 8.
Formulation: lyophilized from a concentrated (1mg/ml) solution containing 5 mM Na3PO4 and 50 mM NaCl, pH 7.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGDPFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR.
Uniprot ACC number: P55075
Transportation method: Shipped at room temperature.
Stability: Lyophilized FGF 8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF 8 should be stored at 4?C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, as determined by its ability to induce proliferation of mouse 3T3 fibroblasts, is 5-7 ng/ml, corresponding to a specific activity of 2x105 units/mg.
Solubility: It is recommended to reconstitute the lyophilized FGF 8 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com