Recombinant human Fibroblast Growth Factor -21 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 182 amino acids and an N-terminal Methionin (bold), having a molecular weight of 19.5 kDa. The FGF-21 is purified by proprietary chromatographic techniques.
Synonyms: Fibroblast growth factor 21, FGF-21.
Formulation: lyophilized from PBS, pH 7.4.
Physical Appearance: Sterile Filtered white Lyophilized powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MHPIPDS SPLLQFGGQV RQRYLYTDDA QQTEAHLEIR EDGTVGGAAD QSPESLLQLK ALKPGVIQIL GVKTSRFLCQ RPDGALYGSL HFDPEACSFR ELLLEDGYNV YQSEAHGLPL HLPGNKSpH R DPAPRGPARF LPLPGLPPAP PEPPGILAPQ PPDVGSSDPL SMVGPSQGRS PSYAS.
Uniprot ACC number: Q9NSA1
Transportation method: Shipped at room temperature.
Stability: Lyophilized FGF-21 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose dependent stimulation of the proliferation of BAF3 cells expressing FGF receptors is 0.06-0.4µg/ml in the presence of betaKlotho.
Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Human Recombinant sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title: Fibroblast Growth Factor 21 Action in the Brain Increases Energy Expenditure and Sensitivity in Obese Rats Publication: Diabetes 59.7 (2010): 1817-1824. Link: http://diabetes. diabetesjournals. org/content/59/7/1817. full
Safety data sheet: Inquire at info@3hbiomedical.com