Recombinant human FGF20 (1-211) produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids and having a molecular mass of 24 kDa. The FGF-20 is fused to a 6 amino acid His-Tag [HHHHHH] at N-terminus and purified by proprietary chromatographic techniques.
Synonyms: Fibroblast Growth Factor 20, FGF-20, RHDA2, FGF20.
Formulation: lyophilized from a 0.2 µm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MHHHHHHAPL AEVGGFLGGL EGLGQQVGSH FLLPPAGERP PLLGERRSAA ERSARGGPGA AQLAHLHGIL RRRQLYCRTG FHLQILPDGS VQGTRQDHSL FGILEFISVA VGLVSIRGVD SGLYLGMNDK GELYGSEKLT SECIFREQFE ENWYNTYSSN IYKHGDTGRR YFVALNKDGT PRDGARSKRH QKFTHFLPRP VDPERVPELY KDLLMYT.
Uniprot ACC number: Q9NP95
Transportation method: Shipped at room temperature.
Stability: Lyophilized FGF20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-20 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 2.5 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized FGF-20 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com