Recombinant human Fibroblast Growth Factor-19 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a molecular mass of 23 kDa. The amino acid sequence of the recombinant human FGF19 is 100% homologous to the amino acid sequence of the human FGF19 without signal sequence and contains His-Tag at N-terminal. The FGF-19 is purified by proprietary chromatographic techniques.
Synonyms: Fibroblast growth factor 19, FGF-19.
Formulation: Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20 mM TRIS, 20 mM NaCl, pH 7.5.
Physical Appearance: Filtered white Lyophilized powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MRGSHHHHHH GMASLAFSDA GpH VHYGWGD PIRLRHLYTS GpH GLSSCFL RIRADGVVDC ARGQSAHSLLEIKAVALRTV AIKGVHSVRY LCMGADGKMQ GLLQYSEEDC AFEEEIRPDG YNVYRSEKHR LPVSLSSAKQ RQLYKNRGFL PLSHFLPMLP MVPEEPEDLR GHLESDMFSS PLETDSMDPF GLVTGLEAVR SPSFEK.
Uniprot ACC number: O95750
Transportation method: Shipped at room temperature.
Stability: Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: Add deionized water to a working concentration approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com