Recombinant human FGF18 produced in
E. coli is a single, non-glycosylated, polypeptide chain (Glu28-Ala207) containing 190 amino acids including a 10 aa His-Tag at N-terminus. The total calculated molecular mass is 22.3 kDa.
Synonyms: Fibroblast growth factor 18, FGF-18, zFGF5, FGF18.
Formulation: FGF18 was filtered (0.4 µm) and Lyophilized in phosphate buffered saline and 5% w/v trehalose.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Purity as determined by densitometric image analysis is greater than 95%.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHASEENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQPELQK PFKYTTVTKR SRRIRPTHPA.
Uniprot ACC number: O76093
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. FGF18 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com