Recombinant human EPOR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 232 amino acids (25-250 a.a.) and having a molecular mass of 25.6 kDa (Molecular size on SDS-PAGE will appear at approximately 28-40 kDa). EPOR is expressed with a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Synonyms: EPO-R, EPOR, Erythropoietin Receptor.
Formulation: EPOR protein solution (0.5 mg/ml) contains phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95% as determined by analysis by SDS-PAGE.
Source: Sf9, Baculovirus cells.
Amino acid sequence: APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDpH HHH HH.
Uniprot ACC number: P19235
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Biological Activity: Measured by its ability to inhibit EPO dependent proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect less or equal to 70 ng/ml.
Safety data sheet: Inquire at info@3hbiomedical.com