Recombinant human EPO-a produced in HEK cells is a glycosylated monomer, having a total molecular weight of 36 kDa. The EPO-Alpha is purified by proprietary chromatographic techniques.
Synonyms: Erythropoietin-Alpha, EPO-a, EPO-Alpha, Epoetin, EP, MGC138142.
Formulation: The EPO-Alpha was lyophilized from 1mg/ml in 1xPBS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Uniprot ACC number: P01588
Transportation method: Shipped at room temperature.
Stability: Lyophilized EPO-Alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-Alpha should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.5-2.5 ng/ml, corresponding to a specific activity of 400,000-2,000,000 units/mg.
Solubility: It is recommended to reconstitute the lyophilized EPO-Alpha in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com