Recombinant human Erythropoietin-Alpha is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21 kDa and apparent glycosylated molecular mass of 36-40 kDa. EPO-a is purified by proprietary chromatographic techniques.
Synonyms: Erythropoietin-Alpha, EPO-a, EPO-Alpha, EP, MGC138142.
Formulation: Each mg of Lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Chinese Hamster Ovary Cells(CHO).
Amino acid sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.
Uniprot ACC number: P01588
Transportation method: Shipped at room temperature.
Stability: Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-Alpha should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized EPO-Alpha in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency1–3Publication:Journal of Nutrition, doi:10.3945/jn.108 .093575Vol.138, No.11, 2076-2083, November 2008© 2008 American Society for Nutrition J. Nutr.138:2076-2083, November 2008Link:http://jn. nutrition. org/content/138/11/2076. full2. Title:Systemically delivered Erythropoietin transiently enhances adult hippocampal neurogenesis. Publication:Article first published online: 7 MAY 2007 DOI:10.1111/j.1471-4159.2007.04684. x Journal of Neurochemistry Volume 102 , Issue 6, pages 1953–1965, September 2007. Link:http://onlinelibrary. wiley. com/doi/10.1111/j.1471-4159.2007.04684. x/full3. Title:ZINC TRANSPORTER EXPRESSION IN MATURE RED BLOOD CELLS andDIFFERENTIATING ERYTHROID PROGENITOR CELLS. Publication:UNIVERSITY OF FLORIDA© 2007 Moon-Suhn RyuLink:http://ufdcimages. uflib. ufl. edu/UF/E0/02/14/46/00001/ryu_m. pdf4. Title:Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Publication:First Published Online May 3, 2012 doi: 10.5966/sctm.2011-0048 Stem Cells Trans Med May 2012 vol.1 no.5 373-383Link:http://stemcellstm. Alphamedpress. org/content/1/5/373. full
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