Recombinant Human EGF Long produced in
E. coli cells is a single non-glycosylated, polypeptide chain containing 106 amino acids and having a molecular mass of 12.3 kDa. The EGF Long is purified by proprietary chromatographic techniques.
Synonyms: Urogastrone, URG, EGF.
Formulation: The EGF Long was lyophilized from a 0.2 µm filtered concentrated solution in 10 mM HCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MFPAMPLSSL FANAVLRAQH LHQLAADTYK EFERAYIPEG QRYSIQVNFA HYGNSDSECP LSHDGYCLHD GVCMYIEALD KYACNCVVGY IGERCQYRDL KWWELR
Uniprot ACC number: P01133
Transportation method: Shipped at room temperature.
Stability: Lyophilized EGF Long although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF Long should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized EGF Long in sterile 100 mM AcOH (acetic Acid) not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com