Recombinant human DHH (C23II) produced in
E. coli is a single, non-glycosylated polypeptide chain containing 177 amino acids and having a molecular mass of 19.9 kDa. The DHH (C23II) is purified by proprietary chromatographic techniques.
Synonyms: HHG-3, Desert Hedgehog homolog, MGC35145, Desert hedgehog protein, DHH.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in 1×PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG.
Uniprot ACC number: O43323
Transportation method: Shipped at room temperature.
Stability: Lyophilized DHH (C23II) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DHH (C23II) should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The Biological Activity was determined by its ability to induce alkaline pH ospH atase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 15-45 μg/ml.
Solubility: It is recommended to reconstitute the lyophilized DHH (C23II) in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com