Recombinant human CTGF produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and having a molecular mass of 11.2 kDa. The CTGF is purified by proprietary chromatographic techniques.
Synonyms: CCN2, NOV2, HCS24, IGFBP8, MGC102 839, CTGF, Connective Tissue Growth Factor.
Formulation: CTGF was lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Purity of CTGF is greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTpH RTTTL PVEFKCPDGE VMKKN mMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA.
Uniprot ACC number: P29279
Transportation method: Shipped at room temperature.
Stability: Lyophilized CTGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTGF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1-2 µg/ml, corresponding to a specific activity of 500-1000units/mg.
Solubility: It is recommended to reconstitute the lyophilized CTGF in sterile 18MΩcm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com