Recombinant human Clusterin produced in HEK is a glycosylated, polypeptide chain containing 438 amino acids and having a molecular mass of 51.27 kDa. Clusterin (1-427 a.a.) is fused to 11 a.a. flag tag at c-terminal and purified by proprietary chromatographic techniques.
Synonyms: CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, Apo-J.
Formulation: Filtered (0.4 micron) and Lyophilized PBS, pH 7.5.
Physical Appearance: Filtered, White, Lyophilized powder.
Purity: Greater than 95% as determined by SDS PAGE.
Source: 293 cell line (Human embryonic kidney).
Amino acid sequence: DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NET mMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRpH FFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEAAA DYKDDDDK.
Uniprot ACC number: P109 09
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
References: 1. Tiltle: Clusterin Facilitates Exchange of Glycosyl pH ospH atidylinositol-Linked SPAM1 Between Reproductive Luminal Fluids and Mouse and Human Sperm Membranes1. Publication:BIOLOGY OF REPRODUCTION 81, 562–570 (2009) Published online before print 8 April 2009. DOI 10.109 5/biolreprod.108 .075739Link:http://www. biolreprod. org/content/81/3/562. full. pdf2. Tiltle: Mass spectrometry quantification of clusterin in the human brain. Publication:2012 Chen et al. ; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the CreativeCo mMons Attribution License (http://creativeco mMons. org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Link:http://link. springer. com/content/pdf/10.1186%2F1750-1326-7-41. pdf
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