Recombinant human Ciliary Neurotrophic Factor Recombinant Human produced in
E. coli is a single, non-glycosylated polypeptide chain (aa 1-200) containing a total of 220 amino acids and having a molecular mass of 25 kDa. The CNTF protein is fused to a 20 aa His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms: HCNTF, CNTF, Ciliary Neurotrophic Factor.
Formulation: CNTF protein solution (1mg/ml) contains 20 mM Tris-HCl buffer pH 8 and 1 mM DTT.
Physical Appearance: Sterile Filtered colorless clear solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT pH RRDLCSRS IWLARKIRSDLTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM.
Uniprot ACC number: P26441
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at info@3hbiomedical.com