Recombinant human CDNF produced in
E. coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.5 kDa. The CDNF is purified by proprietary chromatographic techniques.
Synonyms: Cerebral Neurotrophic factor, arginine-rich, mutated in early stage tumors-like 1, Conserved Neurotrophic factor, ARMET-like protein 1, ARMETL1.
Formulation: CDNF protein was lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L
Uniprot ACC number: Q49AH0
Transportation method: Shipped at room temperature.
Stability: Lyophilized CDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CDNF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by its ability to stimulate the proliferation of rat C6 cells is 15-25 µg/ml, corresponding to a specific activity of 40-67units/mg.
Solubility: It is recommended to reconstitute the lyophilized CDNF in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com