Recombinant human BDNF is a single, glycosylated polypeptide chain containing 116 amino acids (19-128a.a.) and having a molecular mass of 13.1 kDa (calculated). BDNF is fused to a 6 a.a. His-Tag at C-terminal.
Synonyms: Brain-Derived Neurotrophic Factor, BDNF, MGC34632, Abrineurin, ANON2, BULN2.
Formulation: BDNF filtered (0.4 µm) and lyophilized from 0.5 mg/ml in PBS.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: HEK293 Cells.
Amino acid sequence: APMKEANIRG QGGLAYPGVR THGTLESVNG PKAGSRGLTS LADTFEHVIE ELLDEDQKVR PNEENNKDAD LYTSRVMLSS QVPLEPPLLF LLEEYKNYLD AANMSMRVRR HHHHHH.
Uniprot ACC number: P23560
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely.
Safety data sheet: Inquire at info@3hbiomedical.com