Recombinant human Brain-derived Neurotrophic Factor produced in
E. coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids (and an N-terminal Met) and having a total molecular mass of 27 kDa. BDNF is purified by proprietary chromatographic techniques.
Synonyms: Brain-Derived Neurotrophic Factor, BDNF, MGC34632.
Formulation: The protein was Lyophilized without any additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: BDNF is greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.
Uniprot ACC number: P23560
Transportation method: Shipped at room temperature.
Stability: Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2 µg/ml.
Solubility: It is recommended to reconstitute the lyophilized BDNF in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title:Generation of Neurons with Improved Cell Survival and pH enotype Maintenance Using a Degradation-Resistant Nurr1 Mutant†‡Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press. Link:http://onlinelibrary. wiley. com/doi/10.1002/stem.146/full
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