Recombinant human Nerve Growth Factor-beta produced in
E. coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers. The NGF-b is purified by proprietary chromatographic techniques.
Synonyms: Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Formulation: The beta-NGF protein was lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA.
Uniprot ACC number: P01138
Transportation method: Shipped at room temperature.
Stability: Lyophilized Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-Beta should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50, calculated by its ability to stimulate proliferation of TF-1 cells and is typically 1.31 ng/ml
, corresponding to a specific activity of 7.6x105 units/mg.
Solubility: It is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title: u-Opioid receptor activation in live cellsPublication: Journal 22.10 (2008): 3537-3548. Link: http://www. fasebj. org/content/22/10/3537. full
Safety data sheet: Inquire at info@3hbiomedical.com