Recombinant human BD5 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 5.8 kDa. The Beta Defensin-5 is purified by proprietary chromatographic techniques.
Synonyms: Beta-defensin 105 , eta-defensin 5, BD-5, DEFB-5, Defensin, beta 105 , DEFB105 A, BD5, DEFB105 , DEFB5, DEFB105 B.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: GLDFSQPFPS GEFAVCESCK LGRGKCRKEC LENEKPDGNC RLNFLCCRQR I.
Uniprot ACC number: Q8NG35
Transportation method: Shipped at room temperature.
Stability: Lyophilized Beta Defensin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD5 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized Beta Defensin-5 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com