Recombinant human Beta Defensin-2 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.3 kDa. The BD-2 is purified by proprietary chromatographic techniques.
Synonyms: BD-2, hBD-2, Defensin beta 2, Skin-antimicrobial peptide 1, SAP1, DEFB4, DEFB102 , DEFB2, DEFB4P, Beta-defensin 2.
Formulation: The Human BD-2 was lyophilized from a 0.2 µm filtered concentrated (1mg/ml) solution containing 20 mM PB pH 7.4 and 130 mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.
Uniprot ACC number: O15263
Transportation method: Shipped at room temperature.
Stability: Lyophilized Beta Defensin-2 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-2 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Determined by the ability to chemoattract human dendritic i mMature cells at a concentration of 10-100 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized Beta Defensin-2 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com