Recombinant human Beta Defensin-1 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa. The BD-1 is purified by proprietary chromatographic techniques.
Synonyms: Beta-defensin 1, BD-1, Defensin beta 1, hBD-1, HBD1, HBP1, DEFB1, HBD-1, HBP-1, DEFB101, DEFB-1, MGC51822.
Formulation: The Human BD-1 was lyophilized from a concentrated (1mg/ml) solution containing 20 mM PBS pH 7.4 and 130 mM sodium chloride.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK.
Uniprot ACC number: P60022
Transportation method: Shipped at room temperature.
Stability: Lyophilized Beta Defensin-1 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-1 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 100-1000 ng/ml corresponding to a specific activity of 1,000-10,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Beta Defensin-1 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com