Recombinant human BNP produced in
E. coli is a single, non-glycosylated, polypeptide chain (a.a. 27-102) containing 83 amino acids including a 7 a.a. N-terminal His-Tag. The total molecular mass is 9.4 kDa (calculated).
Synonyms: NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.
Formulation: BNP filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20 mM Tris buffer and 50 mM NaCl, pH 7.5.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MKHHHHHHPL GSPGSASDLE TSGLQEQRNH LQGKLSELQV EQTSLEPLQE SPRPTGVWKS REVATEGIRG HRKMVLYTLR APR.
Uniprot ACC number: P16860
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. BNP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com