Recombinant human APRIL produced in
E. coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (105 -247) and having a molecular mass of 17.6 kDa. APRIL is fused to a 16 amino acid T7-tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms: Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, TNF- and APOL-related leukocyte expressed ligand 2, TALL-2, TNF-related death ligand 1, TRDL-1, CD256, TNFSF13, TALL2, ZTNF2, UNQ383/PRO715.
Formulation: APRIL protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0), 0.4 M UREA and 10% glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 85% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MASMTGGQQM GRGSHMAVLT QKQKKQHSVL HLVPINATSK DDSDVTEVMW QPALRRGRGL QAQGYGVRIQ DAGVYLLYSQ VLFQDVTFTM GQVVSREGQG RQETLFRCIR SMPSHPDRAY NSCYSAGVFH LHQGDILSVI IPRARAKLNL SpH GTFLGL.
Uniprot ACC number: O75888
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at info@3hbiomedical.com