Recombinant human Apolipoprotein-D His-Tag fusion protein at C-terminus (7 highlighted a.a.) produced in
E. coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.82 kDa. The protein a.a. sequence corresponds to the UniProtKB/Swiss-Prot entry P05090. The Following gene modifications were made:Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apolipoprotein-D to enhance the protein’s solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchanges which facilitate its genetic manipulation. The Apolipoprotein-D is purified by proprietary chromatographic techniques.
Synonyms: Apolipoprotein D, Apo-D, ApoD.
Formulation: Filtered (0.4 µm) and lyophilized from 1mg/ml in 4 mM KH2PO4, 16 mM Na2HPO4 and 115 mM NaCl pH 7.5.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH.
Uniprot ACC number: P05090
Transportation method: Shipped at room temperature.
Stability: Store Lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: It is recommended to add deionized H
2O to a working volume of 0.5 mg/ml and let the Lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com