Recombinant human ANGPT2 produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain (19-496 a.a.) and fused to a 6 aa His-Tag at C-terminus containing a total of 484 amino acids and having a molecular mass of 55.7 kDa.a.NGPT2 shows multiple bands between 50-100 kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Synonyms: Angiopoietin-2, Angiopoietin2, ANGPT2, ANG2, ANG-2, ANGPT-2.
Formulation: ANGPT2 protein solution (0.25 mg/ml) contains phosphate buffered saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source: Chinese Hamster Ovary Cells (CHO).
Amino acid sequence: YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQRLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQTRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKNSFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQHDLMETVNNL LT mMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEEIKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQRYVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDGDNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT mMIRPADFHH HHHH
Uniprot ACC number: O15123
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at info@3hbiomedical.com