Recombinant human The Acrp30 produced in HEK293 cells is fused with FLAG tag.
Synonyms: Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Formulation: Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 50 mM phosphate Buffer, 75 mM NaCl, pH 7.4.
Physical Appearance: White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by HPLC and SDS PAGE.
Source: HEK293 (Human Embryonic Kidney cell line).
Amino acid sequence: ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK.
Uniprot ACC number: Q15848
Transportation method: Shipped at room temperature.
Stability: For long term, store Lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C. The Lyophilized protein remains stable for 24 months when stored at -20°C.
Biological Activity:in vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from ma mMalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.
Solubility: Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the Lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com