Recombinant human Active form Activin-A produced in
E. coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2 kDa. The Active form Activin-A is purified by standard chromatographic techniques.
Synonyms: Inhba, Inhibin beta A, FSH releasing protein.
Formulation: Human Activin-A was lyophilized from a concentrated 1mg/ml protein solution containing 0.1% TFA.
Physical Appearance: Lyophilized freeze dried powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source:E. coli.
Amino acid sequence: MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS.
Uniprot ACC number: P08476
Transportation method: Shipped at room temperature.
Stability: Lyophilized Activin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Activin-A should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Biological activity is assessed by the ability to induce cytoxicity of MPC-11 cells and was found to be 8.8 ng/ml corresponding to a specific activity of 1.1 x 105 units/mg.
Solubility: Human INHBA protein should be reconstituted in distilled pyrogen free water to a concentration of 100ug /ml which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com