Recombinant human 4-1BB Soluble Receptor Recombinant Human also called Tumor necrosis factor receptor superfamily member 9 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 167 amino acids, having a molecular mass of 17.7 kDa and containing the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor. The 4-1BB Receptor is purified by proprietary chromatographic techniques.
Synonyms: Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor T-cell, antigen 4-1BB homolog, T-cell antigen ILA, CD137 antigen, CDw137, ILA, 4-1BB, MGC2172, 4-1BBR, TNFRSF9.
Formulation: lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Thr-Arg.
Uniprot ACC number: Q07011
Transportation method: Shipped at room temperature.
Stability: Lyophilized 4-1BB Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution 4-1BBR should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The activity was determined by the inhibition of 4-1BB ligand mediated stimulation of IL-8 production by human PBMC. Results: 90% inhibition using 1µg for both 4-1BB ligand and 4-1BB receptor.
Solubility: It is recommended to reconstitute the lyophilized 4-1BB Receptor in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com