Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in
E. coli is a non-glycosylated, Polypeptide chain containing 78 amino acids, having a molecular mass of 9.2 kDa. The SDF-1a is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms: SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.
Formulation: The protein was filtered (0.4 µm) and lyophilized from a concentrated (0.5mg/ml) solution containing 20mM Tris buffer pH 7.5 and 20mM sodium chloride.
Physical Appearance: White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MKHHHHHHAS KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.
Uniprot ACC number: P48061
Transportation method: Shipped at room temperature.
Stability: Lyophilized SDF1A although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Solubility: It is recommended to add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at info@3hbiomedical.com