Macrophage Inflammatory Protein-1 beta Human Recombinant produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7.6 kDa. The CCL4 is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine A4, CCL4, Macrophage inflammatory protein 1-beta, MIP-1- beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, H400, SIS-gamma, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, chemokine (C-C motif) ligand 4, ACT2, G-26, LAG1, MIP1B, SCYA4, AT744.1, MGC104418, MGC126025, MGC126026.
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser.
Uniprot ACC number: P13236
Transportation method: Shipped at room temperature.
Stability: Lyophilized MIP-1b although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title: CONFUSION BETWEEN CCL4 AND CCL4L1: AN EXAMPLE TO BEAR IN MIND WHEN USING THIRD-PARTY REAGENTS. Publication: Inmunologia Vol. 26 / Num 1/ Enero-Marzo 2007: 51-54 (Spanish). Link: http://revista.inmunologia.org/Upload/Articles/7/5/756.pdf
Safety data sheet: Inquire at info@3hbiomedical.com