Macrophage Inflammatory Protein-1 alpha Human Recombinant produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa. The MIP-1a is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
Formulation: The protein was lyophilized from 0.55 mg/ml solution containing no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Leu-Ala-Ala.
Uniprot ACC number: P10147
Transportation method: Shipped at room temperature.
Stability: Lyophilized MIP-1a although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The Activity is calculated by the ability of chemo-attraction of Human monocytes using 1-10 ng/ml corresponding to a Specific Activity of 100,000-1,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com