Epithelial Neutrophil-Activating Protein 78 Human Recombinant produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8 kDa. The CXCL5 is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine B5, CXCL5, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, ENA-78 (1-78), chemokine (C-X-C motif) ligand 5, SCYB5.
Formulation: The CXCL5 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg.
Uniprot ACC number: P42830
Transportation method: Shipped at room temperature.
Stability: Lyophilized ENA78 although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL5 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 100ng/mL (corresponding to a Specific Activity of 10,000 IU/mg) of recombinant human ENA-78.
Solubility: It is recommended to reconstitute the lyophilized ENA-78 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com