Eotaxin Human Recombinant produced in
E. coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.3 kDa. The CCL11 is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine A11, CCL11, Eosinophil chemotactic protein, chemokine (C-C motif) ligand 11, SCYA11, MGC22554.
Formulation: Lyophilized from a 0.2 µm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDICADPKKKWVQD SMKYLDQKSP TPKP.
Uniprot ACC number: P51671
Transportation method: Shipped at room temperature.
Stability: Lyophilized Eotaxin although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL11 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at a concentration between 0.1-10 ng/ml corresponding to a Specific Activity of 100,000-10,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Eotaxin Human Recombinant in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com