Proguanylin produced in
E. coli is a single, non-glycosylated polypeptide chain (a. A 22-115) containing 104 amino acids including a 10 a. A N-terminal His tag. The total molecular mass is 11.5 kDa (calculated).
Synonyms: Guanylin Precursor, Guanylate cyclase activator 2A, Guanylate cyclase-activating protein 1, Gap-IGUCA2, STARA, GUANYLIN.
Formulation: Proguanylin filtered (0.4 µm) and lyophilized in 0.5mg/ml in deionized H20.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MKHHHHHHAS VTVQDGNFSF SLESVKKLKD LQEPQEPRVG KLRNFAPIPG EPVVPILCSN PNFPEELKPL CKEPNAQEIL QRLEEIAEDP GTCEICAYAA CTGC.
Uniprot ACC number: Q02747
Transportation method: Shipped at room temperature.
Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after one week at 4°C.
Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Proguanylin is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Safety data sheet: Inquire at
info@3hbiomedical.com