PTHrP N15 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids, having an MW of 10 kDa labeled by the stable isotope N15. The PTHrP is purified by proprietary chromatographic techniques.
Synonyms: Parathyroid Hormone 2, PTH2, TIPF39, Tuberoinfundibular 39 Residue Protein.
Formulation: PthRp N15 protein was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP.
Uniprot ACC number: Q96A98
Transportation method: Shipped at room temperature.
Stability: Lyophilized PTHrP N15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTHrP N15 should be stored at 4C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.
Safety data sheet: Inquire at
info@3hbiomedical.com