Parathyroid Hormone produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 28 amino acids and having a molecular mass of 3.4 kDa. The PTH is purified by proprietary chromatographic techniques.
Synonyms: PTH/PTHrP receptor antagonist, PTHrP analog, PTHR, MGC138426, MGC138452.
Formulation: The protein (1 mg/ml) was lyophilized after extensive dialyses against 20mM PBS pH-7.0 and 150mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: LMHNLGKHLN SMERVEWLRK KLQDVHNF.
Uniprot ACC number: Q03431
Transportation method: Shipped at room temperature.
Stability: Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 10,000 Units/mg.
Solubility: It is recommended to reconstitute the lyophilized PTH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at
info@3hbiomedical.com