PTH (1-84) N15 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 84 amino acids and having a molecular mass of 9.6 kDa labeled by the stable isotope N15. The PTH (1-84) N15 is purified by proprietary chromatographic techniques.
Synonyms: Parathyrin, PTH, Parathormone.
Formulation: PTH (1-84) N15 protein was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ.
Uniprot ACC number: P01270
Transportation method: Shipped at room temperature.
Stability: Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 9,000 Units/mg.
Solubility: It is recommended to reconstitute the lyophilized Parathormone in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at
info@3hbiomedical.com