The Human Agouti-related protein is created as a recombinant protein with N-terminal fusion of His Tag. The Human Agouti-related protein His-Tagged Fusion Protein, produced in
E. coli, is 14.4 kDa (calculated) protein containing 112 amino acid residues of the human AGRP and 16 additional amino acid residues - His Tag, thrombin cleavage site (highlighted). The AGRP is purified by proprietary chromatographic techniques.
Synonyms: ART, AGRT, ASIP2, MGC118963, AGRP.
Formulation: AGRP protein was lyophilized from 0.5mg/ml in 5mM Tris, 25mM NaCl, pH 7.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Purity of Agouti–related protein recombinant human is >95% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT.
Uniprot ACC number: O00253
Transportation method: Shipped at room temperature.
Stability: Store lyophilized AGRP protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Solubility: Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.
Safety data sheet: Inquire at
info@3hbiomedical.com