Recombinant human CD28 produced in Sf9 Baculovirus cells is a single glycosylated polypeptide chain containing 376 amino acids (19-152 a. a.) and having a molecular mass of 42.4 kDa (Molecular size on SDS-PAGE will appear at approximately 40-57 kDa). CD28 is expressed with a 242 amino acids hIgG-His-Tag at C-Terminus and purified by proprietary chromatographic techniques.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen: CD28, T-cell-specific surface glycoprotein CD28 isoform 1.
Formulation: CD28 protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source: Sf9, Baculovirus cells.
Amino acid sequence: ADPNKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKPLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Uniprot ACC number: P10747
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com