Recombinant human CD18 produced in Sf9 Baculovirus cells is a single glycosylated polypeptide chain containing 686 amino acids (23-700 a. a.) and having a molecular mass of 75.9 kDa (Migrates at 70-100 kDa on SDS-PAGE under reducing conditions). CD18 is expressed with an 8 amino acid His-Tag at C-Terminus and purified by proprietary chromatographic techniques.
Synonyms: Integrin Subunit Beta 2, Integrin, Beta 2 (Complement Component 3 Receptor 3 And 4 Subunit), Complement Component 3 Receptor 3 And 4 Subunit, CD18, MFI7, Integrin, Beta 2 (Antigen CD18 (P95), Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1 (Mac-1) Beta Subunit), Cell Surface Adhesion Glycoprotein (LFA-1/CR3/P150,959 Beta Subunit Precursor), Cell Surface Adhesion Glycoproteins LFA-1/CR3/P150,95 Subunit Beta, Leukocyte-Associated Antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte Cell Adhesion Molecule CD18, Complement Receptor C3 Beta-Subunit, Complement Receptor C3 Subunit Beta, Integrin Beta Chain, Beta 2, CD18 Antigen, LFA-1, MAC-1, LCAMB, MF17, LAD, CD18.
Formulation: CD18 protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 85% as determined by SDS-PAGE.
Source: Sf9, Baculovirus cells.
Amino acid sequence: QECTKFKVSS CRECIESGPG CTWCQKLNFT GPGDPDSIRC DTRPQLLMRG CAADDIMDPT SLAETQEDHN GGQKQLSPQK VTLYLRPGQA AAFNVTFRRA KGYPIDLYYL MDLSYSMLDD LRNVKKLGGD LLRALNEITE SGRIGFGSFV DKTVLPFVNT HPDKLRNPCP NKEKECQPPF AFRHVLKLTN NSNQFQTEVG KQLISGNLDA PEGGLDAMMQ VAACPEEIGW RNVTRLLVFA TDDGFHFAGD GKLGAILTPN DGRCHLEDNL YKRSNEFDYP SVGQLAHKLA ENNIQPIFAV TSRMVKTYEK LTEIIPKSAV GELSEDSSNV VQLIKNAYNK LSSRVFLDHN ALPDTLKVTY DSFCSNGVTH RNQPRGDCDG VQINVPITFQ VKVTATECIQ EQSFVIRALG FTDIVTVQVL PQCECRCRDQ SRDRSLCHGK GFLECGICRC DTGYIGKNCE CQTQGRSSQE LEGSCRKDNN SIICSGLGDC VCGQCLCHTS DVPGKLIYGQ YCECDTINCE RYNGQVCGGP GRGLCFCGKC RCHPGFEGSA CQCERTTEGC LNPRRVECSG RGRCRCNVCE CHSGYQLPLC QECPGCPSPC GKYISCAECL KFEKGPFGKN CSAACPGLQL SNNPVKGRTC KERDSEGCWV AYTLEQQDGM DRYLIYVDES RECVAGPNLE HHHHHH
Uniprot ACC number: P05107
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com